The applications of cell theraphy

The history of the use of artificial tissues as a substitute for compromised tissue is born in the eighties, when they were put in place the conditions to expand the keratinocytes, the cells of the epidermis that is, to reconstruct in the laboratory flaps of tissue to be transplanted into patients who had suffered burns. From a small skin biopsy of the patient are isolated keratinocytes, cultivated in a test tube until the formation of a package that can be transplanted on the surface burned. Other interesting applications have focused on the reconstruction of the corneal epithelium of patients who had undergone thermal or chemical burns to the eye. More recently the interest of scientists turned to the regeneration and repair of skeletal tissues, bone and cartilage, through the use of cells isolated from the bone marrow, respectively, or from a small biopsy of cartilage. In the case of large bone defects, the regenerative ability of the bone is insufficient to ensure the repair of the lesion and the current therapeutic techniques of transplantation of autologous bone or from donor have limitations. Stromal cells derived from bone marrow can in these cases represent a suitable cell type in the regeneration of bone tissue, given their osteogenic potential. There are also numerous studies related to the use of cartilage cells, chondrocytes for the repair of cartilage lesions. The progress achieved by encouraging scientific research have opened new possibilities in the use of tissue engineering for the treatment of diabetes, the regeneration of cardiac muscle tissue when the heart is damaged by a heart attack and the use of endothelial cells for the coating of implants vascular. Even the nerve cells destroyed by degenerative diseases such as Parkinson's disease and Alzheimer's disease could be replaced by the use of cell therapy.

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Construction of complex tissues

The development of knowledge of cell biology integrated with those of bioengineering has recently opened new perspectives in the field of tissue engineering has established itself, which proposes the use of the cells in the laboratory for the construction of artificial biological tissues to be used as a substitute for damaged ones in result of disease or trauma. This would help solve the problems of reduced availability of organs for transplantation and the risk of graft rejection derived from the donor. The association of cells with biocompatible and biodegradable materials is leading to the production of engineered tissues transplantable. The actors involved in the scenario biotech tissue regeneration are basically three: the cells, signaling molecules and biomaterials .

To ensure the maintenance of the function of the implanted cells using a material or scaffold that acts as a scaffold to guide the three-dimensional organization of the cells in the final construction. The scaffold , synthetic or natural, must be able to support the growth and differentiation of cells, ie it must recreate the more possible the microenvironment characteristic of the fabric that you want to replace. Once transplanted, it will perform the function of guide in tissue growth, before being completely resorbed. For the preparation of the plant to be transplanted, the cells are derived from a fragment of the healthy tissue of the patient to which you want to reconstruct the damaged tissue; follows the combination between the cells and biomaterial appropriate, in the presence or absence of signal molecules necessary for proper cell differentiation. Recently aroused great excitement in the scientific community as a resource in the stem cell regenerative therapy due to their ability to give rise to different cell types depending on how they are stimulated.

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Cell differentiation

The process by which a relatively non-specialized cell becomes a very specialized is called 'differentiation' and affects both the embryo is an individual adult. During embryonic development, many cell types are generated from the fertilized egg cell, to get the range of cells that characterize the adult subject. The route followed by each embryonic cell differentiation is fundamentally dependent on the signals it receives from the environment; the latter, in turn, depend on the position of the cell in the embryo. As a result of the differentiation of different cell types acquire different shapes and express specific proteins: for example, the skeletal muscle cells contain contractile proteins particular, plasma cells are specialized for the production of antibodies, etc.. The differentiation processes continue in the adult body in those tissues that are subject to continuous renewal (eg., Hematopoietic cells).

Each fabric is renewed with its characteristic rhythm, thanks to the presence of stem cells undifferentiated which help keep constant the number of cells in the tissue. Stem cells are defined as undifferentiated cells capable of self-renewal, that is, to produce cells similar to themselves and to generate cells destined to differentiate. They therefore represent a reservoir of cells which the body draws to ensure the renewal of cell tissue death. An example of a stem cell is provided by the hematopoietic stem cells of the bone marrow, capable of giving rise to all cell types of the blood. The adult contains different types of stem cells, which give rise to the cells of the organs in which they are located. Recent studies have however shown that certain adult stem cells have plasticity, ie they are able to differentiate in cell types other than those of the tissue of origin.

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Cell division

Most cell undergoes division, ie, the formation of two daughter cells identical to the parent cell. This event occurs at the end of the cell cycle, which represents the set of modifications that must be met from the moment in which a cell is formed at the moment in which it divides into two daughter cells. The division is preceded by stages in which the cell doubles its intracellular content. Based on the ability to divide, cells are divided into three categories: ( a ) cells in continuous division, as the skin cells, which have a short life and must be replaced quickly; ( b ) stable cells, ie cells that after differentiation entering a phase of rest, from which they can return to the cycle if properly stimulated (eg., hepatocytes); ( c ) perennial cells, ie cells that after differentiation out permanently from the cycle (eg., neurons).  
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The rate of cell division is controlled by mechanisms partially known, that allow a cell to divide only if you need more cells. Numerous specific protein factors regulate the cell cycle, such as hormones and growth factors. The cell acts as a target, thanks to the presence of recognition systems of the stimulus to the division. Normally the number of cycles of replication is related inversely to the age of the animal from which the tissue was taken. For this reason, the cells derived from embryonic tissues can replicate in vitro for a longer time compared to cells isolated from tissues of adult organisms. The cells often lose the ability to divide after a certain period of time; this period is variable for the different cell types and to human cells is approximately 50 divisions. This was demonstrated in 1961 by Leonard Hayflick and Paul S. Moorhead, who observed that human fibroblasts died after a finite number of divisions in culture.

From healthy cell to the transformed cell

Some cells derived from multicellular organisms have a capacity of unlimited division in culture and can be used for the production of cell lines. The cell line is composed of transformed cells derived from tumor tissues or cells in primary culture have been changed as a result of spontaneous mutations or induced by exposure to viruses, chemical mutagens or radiation. Keep in mind that the transformed cells have many unusual characteristics that differentiate them from healthy cells. The alterations that occur as a result of processing related to the presence of a genetic aberrant, the reduction and the alteration of the cellular skeleton ( cytoskeleton ), modifications on the cell surface about the expression of antigens, the lower adhesiveness to the substrate and then the ' increased ability to proliferate in suspension and form multiple layers in the plate. Furthermore, the transformed cells have a lower dependence on the presence of serum in the culture medium and in some cases can produce tumors if they are injected into a suitable host organism.

In 1951, George O. Gey and his colleagues cultured the first human cell line, stabilized by a biopsy of the tumor tissue of the uterine cervix taken from a patient named Henrietta Lacks. The patient died shortly afterwards but his cancer cells were maintained in culture until today. These cells were called HeLa in their name and are currently used in many laboratories. We can obtain continuous cell lines through the introduction in normal cells of specific genes ( oncogenes ) by viral infection. If the tumor virus is added to the culture medium appear in a short time, small colonies of transformed cells that proliferate abnormally.

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How to prepare a cell culture

The first stage in the preparation of a cell culture consists in isolating the desired population of cells from a tissue fragment; in an initial mechanical disaggregation of the tissue is followed by enzymatic digestion, to degrade the extracellular matrix that surrounds and holds the cells together. The resulting cell population is heterogeneous, but using selective media or by separating the cells based on the molecules expressed on the cytoplasmic membrane (antigens), it is possible to isolate specific cell populations. The isolated cells are then grown in an appropriate culture medium; these proliferate and reached confluence, can be detached and moved to another container to keep them in constant division. The cells can be grown to high density (mass culture) or low density (clonal culture), giving rise to the formation of single colonies.

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The culture of plant cells

The techniques for the cultivation of plant cells were developed in the fifties of the twentieth century., When he began to realize that the crops have the potential to produce a wide range of molecules useful in various areas, and currently many types of plant cells can be grown in test tubes. Plant cells are surrounded by a cell wall rather rigid, consisting mainly of cellulose, which gives them a mechanical support, the shape and a permeability barrier. To them we must cultivate the cells treated with an enzyme called 'cellulase', which degrades the cellulose wall of the cell releasing 'naked', also called 'protoplast'. Protoplasts are grown in a culture medium with defined chemical composition, which supports the growth and division. The soil must contain between ingredients also plant hormones, such as auxins, essential for cell division . The cultured cells multiply and form a mass of undifferentiated cells said 'callus', from which, by appropriate stimulation, can originate roots, stalks, leaves and even a whole plant. The pharmaceutical industry uses vegetable crops in the production of anti-cancer drugs, anti-inflammatory, antibacterial; the agricultural sector for the production of pesticides; the food industry with regard to the synthesis of additives (eg., dyes).

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How to feed the cells in Tissue culture

In the living cells remain viable thanks to the contribution of nutrients, guaranteed by the vascular system and, via the capillary network, nourishes the tissue at the cellular level and removes waste products derived from cellular metabolism . In vitro functions vascular vicariate are from the culture medium, a liquid medium highly nutritious. It consists of basic substances, such as glucose, amino acids, vitamins, minerals and trace elements present, necessary for the normal physiological functions of the cell, and from animal serum (usually fetal bovine serum), which supports the growth and proliferation phone. The whey is in most cases used at a concentration of 5 to 20% and contains growth factors, such as platelet growth factor (PDGF), epidermal growth factor (EGF), insulin-like growth factors (IGF) , hormones (eg., insulin), lipids (fatty acids, phospholipids, lecithin and cholesterol) are important as a source of energy and for the synthesis of the plasma membrane and adhesive factors, such as fibronectin and vitronectin, transferrin, important for the metabolism of iron, and albumin, capable of transporting vitamins and lipids. The cells should be fed regularly under aseptic conditions to ensure its viability. Normal cells adhere to surfaces of glass or plastic treated and proliferate to form a confluent monolayer that completely covers the surface of the container (petri dish or flask). To ensure the maintenance of the cells in a microenvironment similar to native cells in the laboratory are kept in incubator at 37 ° C with controlled atmosphere (95% air, 5% CO 2 ), which enables it to maintain the proper pH phone. Depending on the tissue of origin, the cells may require the addition to the culture medium of specific factors, in order to maintain the same degree of proliferation and differentiation.

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